CONFOCAL MICROSCOPE OR PHOTOMETRIC ANALYSIS DEVICE USING OPTICAL SYSTEM OF MULTIPHOTON MICROSCOPE, AND PHOTOMETRIC ANALYSIS METHOD

• Main Author: YAMAGUCHI MITSUSHIRO
• Format: Patent
• Language: English; Japanese
• Published: 23.10.2014
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS; TESTING

PROBLEM TO BE SOLVED: To increase the detected number of light-emitting particles per unit time by the enlargement of a confocal volume in such a manner that an increase of background light intensity can be suppressed as much as possible for measuring a specimen solution low in light emitting particle density in an optical analysis technology using an optical system of a confocal microscope or a multiphoton microscope.SOLUTION: In the optical analysis technology, a division optical system for forming at least two optical detection areas of the optical system in a specimen solution is provided in the optical system of the microscope. Thus, when a cubic volume of the confocal volume is enlarged by increasing the number of confocal volumes, an increase of a background light intensity can be suppressed further than a case where a diameter of one confocal volume is increased to enlarge the cubic volume of the confocal volume. 【課題】 共焦点顕微鏡又は多光子顕微鏡の光学系を用いた光分析技術に於いて、発光粒子濃度の低い試料溶液の計測のために、背景光強度の増大をできるだけ抑制できる態様にてコンフォーカル・ボリュームの拡大による単位時間当たりの発光粒子の検出数の増大を図ること。【解決手段】 本発明の光分析技術では、顕微鏡の光学系に於いて、試料溶液内に光学系の光検出領域を少なくとも二つ形成する分割光学系が設けられる。これにより、コンフォーカル・ボリュームの数を増やすことによりコンフォーカル・ボリュームの体積を拡大すると、一つのコンフォーカル・ボリュームの径を増大してコンフォーカル・ボリュームの体積を拡大する場合よりも背景光強度の増大を抑制できる。【選択図】図２