REAGENTS USEFUL FOR SYNTHESIZING RHODAMINE-LABELED OLIGONUCLEOTIDES

• Main Authors: BENSON SCOTT C, KENNEY PAUL M, ZOU RUIMING N, UPADHYA KRISHNA G, CASSEL JONATHAN M
• Format: Patent
• Language: English
• Published: 08.05.2014
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide reagents useful for synthesizing rhodamine-labeled oligonucleotides.SOLUTION: The disclosure provides reagents that can be used to label synthetic oligonucleotides with rhodamine dyes or dye networks that contain rhodamine dyes. The disclosure provides reagents useful for labeling synthetic oligonucleotides with labels comprising rhodamine dyes that show fluorescence when irradiated with incident light of an appropriate wavelength. The labels can comprise a single rhodamine dye, or they can comprise a dye network in which at least one of a plurality of dyes is a rhodamine dye. The reagents can be used to label synthetic oligonucleotides with rhodamine-containing labels directly during the step-wise synthesis of the oligonucleotides, thereby reducing the manipulation steps necessary to obtain oligonucleotides that are labeled with rhodamine dyes.