TRANSGENIC ANIMAL FOR PRODUCING SPECIFIC ISOTYPES OF HUMAN ANTIBODIES VIA NON-COGNATE SWITCH REGIONS

PROBLEM TO BE SOLVED: To provide fully human antibodies in a transgenic animal of a desired isotype in response to immunization with virtually any desired antigen.SOLUTION: The human immunoglobulin heavy chain transgene in animals includes a human constant region gene segment comprising exons encodi...

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Bibliographic Details
Main Authors IVANOV VLADIMIR E, GREEN LARRY L, DAVIS C GEOFFREY
Format Patent
LanguageEnglish
Published 16.01.2014
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Summary:PROBLEM TO BE SOLVED: To provide fully human antibodies in a transgenic animal of a desired isotype in response to immunization with virtually any desired antigen.SOLUTION: The human immunoglobulin heavy chain transgene in animals includes a human constant region gene segment comprising exons encoding a desired heavy chain isotype, and is operably linked to switch regions from a constant region of a different heavy chain isotype. This additional constant region segment includes a switch region and human constant region coding segment, wherein the constant region coding segment is operably linked to a switch region that is not normally associated with. In the transgene, the non-cognate switch region is a switch region from a different species than the constant region coding segment. The switch region and membrane exons may include a human γ-2 constant region and the secreted constant region exons are from a human γ-1 or a human γ-4 constant region.
Bibliography:Application Number: JP20130202875