METHOD AND COMPOSITION FOR PRODUCING ORTHOGONAL tRNA-AMINOACYL tRNA SYNTHETASE PAIR

• Main Authors: WANG LEI, MAGLIERY THOMAS J, LIU DAVID R, MEHL RYAN AARON, SANTORO STEPHEN WILLIAM, ANDERSON JOHN CHRISTOPHER, ZHANG ZHIWEN, SCHULTZ PETER, CHIN JASON W, MEGGERS ERIC L, PASTRNAK MIRO
• Format: Patent
• Language: English
• Published: 26.12.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide more efficient and effective methods to alter and improve the biosynthetic machinery of a cell.SOLUTION: This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. The compositions include new orthogonal tRNA-aminoacyl-tRNA synthetase pairs. The new orthogonal pairs can be used to incorporate unnatural amino acids into proteins in vivo.