VECTOR FOR DIRECTIONAL CLONING

PROBLEM TO BE SOLVED: To provide vectors and methods for directional cloning.SOLUTION: A method of inducing expression of a DNA sequence of interest in a host cell includes a step of contacting a recombinant host cell, which is deficient in rhamnose catabolism and has a recombinant DNA molecule comp...

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Bibliographic Details
Main Authors SLATER MICHAEL R, WOOD KEITH V, HARTNETT JAMES ROBERT, STRAUSS ETHAN EDWARD
Format Patent
LanguageEnglish
Published 12.12.2013
Subjects
BEER
BIOCHEMISTRY
CALCULATING
CHEMISTRY
COMPOSITIONS THEREOF
COMPUTING
COUNTING
CULTURE MEDIA
ELECTRIC DIGITAL DATA PROCESSING
ENZYMOLOGY
INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide vectors and methods for directional cloning.SOLUTION: A method of inducing expression of a DNA sequence of interest in a host cell includes a step of contacting a recombinant host cell, which is deficient in rhamnose catabolism and has a recombinant DNA molecule comprising a rhamnose-inducible promoter operably linked to an open reading frame for a heterologous RNA polymerase, with rhamnose and an expression vector comprising a promoter for the heterologous RNA polymerase operably linked to the DNA sequence of interest.
Bibliography:Application Number: JP20130168742