METHOD FOR PRODUCING Fim3 OF BORDETELLA PERTUSSIS, AND METHOD FOR DETECTING PERTUSSIS

• Main Authors: WAYAMA YUKIMASA, WATANABE MINEO
• Format: Patent
• Language: English
• Published: 28.06.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for detecting pertussis which can distinguish a vaccinee and a pertussis infected person, and is excellent in detection accuracy.SOLUTION: A method of producing Fim3 of Bordetella pertussis is a production method which performs under the absence of a protease in all steps, wherein Bordetella pertussis or a recombinant cell which expresses a pilus of Bordetella pertussis is subjected to a dissolving operation in an aqueous solvent, then an insoluble fraction of the cell separated, subsequently, a solubilization operation of the insoluble fraction is performed, a purification operation is performed while contacting a solubilized fraction obtained by this to urea to obtain the Fim3. In addition, the Fim3 is used as an antigen, in a specimen, detecting of both sides of an anti-Fim2 antibody and an anti-Fim3 antibody is performed, or detecting of the anti-Fim3 antibody is performed for each anti-pilus antibody, and detecting pertussis in a specimen is performed.