CENTRIFUGING CONTAINER

PROBLEM TO BE SOLVED: To remove an erythrocyte from inside a cell originating from a living tissue, which is obtained from a cell suspension by concentration separation, and thus to perform the concentration separation of the cell originating from the living tissue which has high concentration.SOLUT...

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Bibliographic Details
Main Authors TAKAYAMA MICHIO, KINOSHITA AKI, TOGASHI KYOKO, KIMURA MASATO
Format Patent
LanguageEnglish
Published 19.04.2012
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Summary:PROBLEM TO BE SOLVED: To remove an erythrocyte from inside a cell originating from a living tissue, which is obtained from a cell suspension by concentration separation, and thus to perform the concentration separation of the cell originating from the living tissue which has high concentration.SOLUTION: A centrifuging container 1 provided herein includes a tubular container body 2 which has a bottom 2a closed in a tapering shape at one end and an inlet 5 of the cell suspension A at the other end, a first partition wall 3 which partitions the inside of the container body 2 into a bottom side space 7 and an inlet side space 8 in the longitudinal direction, and a second partition wall 4 which partitions the bottom side space 7 into a first space 11 being along the inside surface of the container body 2 and a second space 12 being inside the first space 11. The first partition wall 3 is provided with a main channel 9 of a caliber making the inlet side space 8 and the second space 12 communicate with each other and making the flowing cell suspension A produce a Fahraeus-Lindqvist effect, and with branch channels 10a and 10b making a flow near a wall surface branch at a middle position of the main channel 9 and making it communicate with the first space 11.
Bibliography:Application Number: JP20100222667