NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYZER, AND NUCLEIC ACID ANALYSIS METHOD

• Main Authors: HAMAZAKI TAKANOBU, SAITO TOSHIRO
• Format: Patent
• Language: English
• Published: 22.03.2012
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To solve the following problem: in order to arrange micro nucleic acid probes one by one highly densely, a method for having only one molecule of nucleic acid probe captured by a bulky fine particle in advance and selectively fixing the fine particle to a structure arranged arbitrarily is very effective; however, although from viewpoint of fixation response, a fine particle diameter and an adhesion pad had better be large, a fine particle had better be small from viewpoint of excitation, so that a fine particle diameter and an adhesion pad diameter are limited to that extent and an optimal condition for each of fixation response and excitation cannot be met.SOLUTION: An external stimuli-responsive fine particle is used as a bulky fine particle. First, an external stimuli-responsive fine particle which holds one molecule of nucleic acid sample piece is highly densely and regularly arranged on a substrate one by one, external stimulus is given, and volume of the fine particle is reduced.