TRANSGENIC ANIMAL FOR PRODUCING SPECIFIC ISOTYPE OF HUMAN ANTIBODY VIA NON-COGNATE SWITCH REGION

• Main Authors: IVANOV VLADIMIR E, GREEN LARRY L, DAVIS C GEOFFREY
• Format: Patent
• Language: English
• Published: 06.05.2011
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide fully human antibodies in a transgenic animal of a desired isotype in response to immunization with virtually any desired antigen. SOLUTION: The human immunoglobulin heavy chain transgene in animals comprises a human constant region gene segment comprising exons encoding the desired heavy chain isotype, and is operably linked to switch segments from a constant region of a different heavy chain isotype. The additional constant region segment comprises a switch region and a human constant region coding segment, wherein the constant region coding segment is operably linked to the switch region that is not normally associated. In the transgenes, a non-cognate switch region may be a switch region from a different species than the constant region coding segment. The switch region and membrane exons may comprise a human γ-2 constant region and the secreted constant region exons are from a human γ-1 or a human γ-4 constant region. COPYRIGHT: (C)2011,JPO&INPIT