TRANSGENIC ANIMAL FOR PRODUCING SPECIFIC ISOTYPE OF HUMAN ANTIBODY VIA NON-COGNATE SWITCH REGION
PROBLEM TO BE SOLVED: To provide fully human antibodies in a transgenic animal of a desired isotype in response to immunization with virtually any desired antigen. SOLUTION: The human immunoglobulin heavy chain transgene in animals comprises a human constant region gene segment comprising exons enco...
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Main Authors | , , |
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Format | Patent |
Language | English |
Published |
06.05.2011
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Subjects | |
Online Access | Get full text |
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Summary: | PROBLEM TO BE SOLVED: To provide fully human antibodies in a transgenic animal of a desired isotype in response to immunization with virtually any desired antigen. SOLUTION: The human immunoglobulin heavy chain transgene in animals comprises a human constant region gene segment comprising exons encoding the desired heavy chain isotype, and is operably linked to switch segments from a constant region of a different heavy chain isotype. The additional constant region segment comprises a switch region and a human constant region coding segment, wherein the constant region coding segment is operably linked to the switch region that is not normally associated. In the transgenes, a non-cognate switch region may be a switch region from a different species than the constant region coding segment. The switch region and membrane exons may comprise a human γ-2 constant region and the secreted constant region exons are from a human γ-1 or a human γ-4 constant region. COPYRIGHT: (C)2011,JPO&INPIT |
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Bibliography: | Application Number: JP20100272134 |