ISOLATION OF NUCLEIC ACID

• Main Author: BAKER MATTHEW JOHN
• Format: Patent
• Language: English
• Published: 29.07.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for extracting nucleic acids and other biomolecules from blood. SOLUTION: The method for extracting nucleic acids from blood comprises a step to bring blood cells (preferably after lysing the cell) into contact with an activated solid phase at a pH and immobilize the nucleic acid, and a step to remove the nucleic acids at a higher pH when the charge is inverted or neutralized. The solid phase may be a glass bead activated by histidine acting as a binder. The bead is flowable by sucking blood through a column containing the bead with air thereby improving contact of the cell and preventing clogging of the column. COPYRIGHT: (C)2010,JPO&INPIT