ISOLATION OF NUCLEIC ACID

PROBLEM TO BE SOLVED: To provide a method for extracting nucleic acids and other biomolecules from blood. SOLUTION: The method for extracting nucleic acids from blood comprises a step to bring blood cells (preferably after lysing the cell) into contact with an activated solid phase at a pH and immob...

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Bibliographic Details
Main Author BAKER MATTHEW JOHN
Format Patent
LanguageEnglish
Published 29.07.2010
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a method for extracting nucleic acids and other biomolecules from blood. SOLUTION: The method for extracting nucleic acids from blood comprises a step to bring blood cells (preferably after lysing the cell) into contact with an activated solid phase at a pH and immobilize the nucleic acid, and a step to remove the nucleic acids at a higher pH when the charge is inverted or neutralized. The solid phase may be a glass bead activated by histidine acting as a binder. The bead is flowable by sucking blood through a column containing the bead with air thereby improving contact of the cell and preventing clogging of the column. COPYRIGHT: (C)2010,JPO&INPIT
Bibliography:Application Number: JP20100050067