METHOD OF FORMING CALIBRATION CURVE FOR QUANTITATION ASSAY IN INFRARED SPECTROSCOPY AND QUANTITY DETERMINATION METHOD

• Main Authors: HIRAO KEIJI, MIYATAKE KENICHIRO
• Format: Patent
• Language: English
• Published: 27.05.2010
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

PROBLEM TO BE SOLVED: To obtain a highly reliable calibration curve for a quantitation assay in infrared spectroscopy. SOLUTION: The method comprises a preliminary solution preparation step of preparing two or more preliminary solutions 10 with different known contents of a solute dissolved in a solvent, a preliminary sample preparation step of supplying each preliminary solution 10 to a sample stage 20 and then removing the solvent to remain the solute on the stage, a preliminary spectroscopy step of irradiating the solute 11 remaining on the stage with infrared light 40 and taking an infrared absorption spectrum of transmitted or reflected light from the solute, and a calibration curve formation step of obtaining a reciprocal functionality between the known content of the solute in each preliminary solution and specific information of the solute in the infrared absorption spectrum. The sample stage 20 comprises a base 21 having a sample space 22 to define the area where the preliminary solution 10 extends into a certain range and a solute dispersing member (particles 23) having many pores in which the supplied preliminary solution 10 infiltrates when it is disposed within the sample space 22. COPYRIGHT: (C)2010,JPO&INPIT