METHOD FOR QUANTIFYING OR DETECTING DNA

• Main Authors: TOMIGAHARA YOSHITAKA, TARUI HIROKAZU, SATO HIDEO
• Format: Patent
• Language: English
• Published: 02.04.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for simply quantifying or detecting a DNA. SOLUTION: The method for quantifying or detecting a methylated DNA in the objective DNA region possessed by a genomic DNA contained in a specimen derived from an organism is provided. The method and the like include: a first step of preparing a test oligonucleotide which is a DNA having the plurality of objective DNA regions present in the genome from the specimen; and a second step etc., of mixing the test oligonucleotide prepared in the first step with a detecting oligonucleotide complementarily binding to the test oligonucleotide, a specific oligonucleotide complementarily binding to the test oligonucleotide, and a support binding to the specific oligonucleotide, and forming a detection complex composed of the test oligonucleotide, detecting oligonucleotide, specific oligonucleotide, and support. COPYRIGHT: (C)2010,JPO&INPIT