EXPRESSION VECTOR AND METHOD FOR USING THE SAME

• Main Authors: BLAZEVIC VESNA, MAENNIK ANDRES, USTAV ENE, KROHN KAI, TAEHTINEN MARJA, TOOTS URVE, RANKI ANNAMARI, USTAV MART
• Format: Patent
• Language: English
• Published: 25.02.2010
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a new vector, and a DNA vaccine and a gene therapeutic containing the vector, to provide methods for producing the vector, and the DNA vaccine and the gene therapeutic containing the vector, and to provide a method for therapeutically using the vector. SOLUTION: The vector comprises (a) an expression cassette of a gene of a nuclear-anchoring protein, which contains (i) a DNA binding domain bindable to a specific DNA sequence and (ii) a functional domain bindable to a nuclear component, (b) a multimerized DNA forming a binding site for the nuclear-anchoring protein, and (c) one or more expression cassettes of a DNA sequence of interest. In particular, the vector lacks a papilloma virus origin of replication and a functional replication origin in a mammal cell. The nuclear-anchoring protein includes the E2 protein of bovine papilloma virus type 1 or E2 protein of Epstein-Barr virus nuclear antigen 1. The method for expressing a DNA sequence of interest in a subject is also provided. COPYRIGHT: (C)2010,JPO&INPIT