METHOD FOR SELECTIVELY PRODUCING 1-PHOSPHORYLATED SACCHARIDE DERIVATIVE ANOMER AND METHOD FOR PRODUCING NUCLEOSIDE

• Main Authors: ANDO TOMOYUKI, NAKAMURA TAKESHI, FUJIWARA JUNYA, AWANO HIROICHI, ITO KIYOSHI, IKEDA ICHIRO, MARUYAMA KYOKO, MIYAKE JINKI, UMETANI HIDETAKE, TSUCHIYA KATSUTOSHI, KOMATSU HIRONORI, FUKAZAWA NOBUYUKI, ARAKI TADASHI, ASANO TAMOTSU, YAMAUCHI TAKAHIRO
• Format: Patent
• Language: English
• Published: 12.02.2010
• Subjects: ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HETEROCYCLIC COMPOUNDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for producing an anomer-selective 1-phosphorylated saccharide derivative and a nucleoside, effected with neither skeletal difference, such as furanose and pyranose, existence of substituents, such as deoxysugar, nor kinds of sugar, such as natural type and nonnatural type, and excellent in versatility. SOLUTION: A desired isomer is selectively produced by phosphorolyzing and isomerizing an anomer mixture of a 1-phosphorylated saccharide derivative while crystallizing one of the isomers to displace the equilibrium. Furthermore, by using the action of a nucleoside phosphorylase, a nucleoside is produced from the obtained 1-phosphorylated saccharide derivative and a base with improved stereoselectivity and a high yield. COPYRIGHT: (C)2010,JPO&INPIT