METHOD OF ANALYSIS BY USING NUCLEIC ACID MICROARRAY

• Main Authors: UJIHARA MASARU, KANEHARA HIDEYUKI, INASAWA JOJI, IMOTO TOSHINARI
• Format: Patent
• Language: English
• Published: 14.01.2010
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method of analysis by using a nucleic acid microarray, capable of detecting the change of data originated from the unevenness of amounts of probes, etc., among spots. SOLUTION: This method of analysis by using the nucleic acid microarray having a spot (X1) where a first probe nucleic acid is immobilized, is provided by comprising: a process of hybridizing the labeled sample nucleic acid (A1) of a specimen with the probe nucleic acid immobilized at the spot (X1); a process of imparting a labeled verification nucleic acid (B) having a sequence capable of hybridizing with at least the part of the probe nucleic acid in all of the spots and labeled with a different label from the above labeled sample nucleic acid, to the spot (X1) and hybridizing, a process of measuring the labelled quantity value (F1) of the labeled sample nucleic acid hybridized at the spot (X1); and a process of measuring the labeled quantity value (Fc1) of the labeled verification nucleic acid (B) hybridized on the spot (X1). COPYRIGHT: (C)2010,JPO&INPIT