METHOD FOR PREPARING ANTIBODY-PRODUCING CELL

PROBLEM TO BE SOLVED: To provide a method for stably preparing a monoclonal antibody by establishing a means capable of reproducibly obtaining B cells which has acquired antibody-producing ability. SOLUTION: Provided is a method for preparing an antibody-producing cell which produces a mouse monoclo...

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Bibliographic Details
Main Authors KATO IKUNOSHIN, SANO YUKA, KAMIHAGI KYOKO
Format Patent
LanguageEnglish
Published 10.12.2009
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a method for stably preparing a monoclonal antibody by establishing a means capable of reproducibly obtaining B cells which has acquired antibody-producing ability. SOLUTION: Provided is a method for preparing an antibody-producing cell which produces a mouse monoclonal antibody wherein the method is featured by collecting a tumid lymph node obtained from a mouse immunized by administering an antigen to the neck or back and by using cells obtained from the lymph node for cell fusion. Since the lymph nodes of each part of the mouse are tumefied by the immunization method, these lymph nodes are available as a source of B cell. COPYRIGHT: (C)2010,JPO&INPIT
Bibliography:Application Number: JP20080137882