COMPOSITION FOR DIAGNOSING COLORECTAL CANCER

• Main Authors: LIN SHIU-RU, WANG JAW-YUAN
• Format: Patent
• Language: English
• Published: 02.07.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a composition for diagnosing colorectal cancer for making an early clinical diagnosis of the colorectal cancer, and achieving effect of high specificity, sensitivity, and safety. SOLUTION: A genome sequence is obtained by processes of a. extracting an epithelial cell of intestine of a normal human, and comparing with a tissue of colon polyp and a tissue of colon cancer respectively, b. searching a gene cluster with apparent difference by a suppression subtractive hybridization method, and creating a database, c. extracting a colony which is relatively prominently expressed in a cancer tissue by a colony hybridization method, d. further collecting many clinical cancer tissues, and establishing a change of difference expression of a gene by northern blot, real-time PCR reaction, and bioinformatics analysis, e. selecting an optimum gene cluster and generating the genome sequence in a reagent. COPYRIGHT: (C)2009,JPO&INPIT