PRETREATMENT METHOD FOR DETECTING ACID-FAST BACTERIAL GENE, PRETREATMENT APPARATUS AND METHOD FOR DETECTING GENE

• Main Authors: YOKOTA NAO, MIYAZAKI KOJI
• Format: Patent
• Language: English
• Published: 14.05.2009
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a pretreatment method for detecting an acid-fast bacterial gene contained in expectorated sputum, which is quick and accurate, has high yield, will not cause scattering or contamination of liquid droplets in a closed system, and which isolates acid-fast bacteria from expectorated sputum, in a ready and safe manner, and to provide a method for detecting an acid-fast bacterial gene which includes treating acid-fast bacteria by the pretreatment method, extracting a gene that specifically amplifies and detects the gene. SOLUTION: The pretreatment method for detecting an acid-fast bacterial gene, contained in expectorated sputum includes adding a solubilizing agent of expectorated sputum and a treatment agent for removal of various germ to expectorated sputum, and directly binding/collecting acid-fast bacteria by a solid substrate or a nonspecific ligand which is immobilized on the solid substrate without having to remove the treatment agent. COPYRIGHT: (C)2009,JPO&INPIT