METHOD FOR DETECTING TARGET DNA

PROBLEM TO BE SOLVED: To provide a method for easily and quickly detecting a target DNA at a low cost. SOLUTION: The method for detecting a target DNA includes a first reaction including a step of hybridizing a chimera probe 30 to the target DNA 10, to form a hybrid chain, and a step of cleaving the...

Full description

Saved in:
Bibliographic Details
Main Author TAKUBO TAKESHI
Format Patent
LanguageEnglish
Published 12.03.2009
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:PROBLEM TO BE SOLVED: To provide a method for easily and quickly detecting a target DNA at a low cost. SOLUTION: The method for detecting a target DNA includes a first reaction including a step of hybridizing a chimera probe 30 to the target DNA 10, to form a hybrid chain, and a step of cleaving the hybrid chain with a ribonuclease, to isolate a fragment of the chimera probe, and a second reaction including a step of hybridizing the fragment to a single stranded circular DNA, a step of extending the fragment with a strand displaced DNA polymerase and a step of detecting the extended fragment. COPYRIGHT: (C)2009,JPO&INPIT
Bibliography:Application Number: JP20070221341