NEW PROTEIN AND METHOD FOR PRODUCING THE SAME

• Main Authors: WASHIDA NAOHIRO, TOO KANJI, TSUDA HIDEYORI, YANO KAZUKI, SHIMA NOBUYUKI, KINOSAKI MASAHIKO, TOMOYASU MASAHIRO, NAKAGAWA NOBUAKI, TAKAHASHI KEN, YASUDA NAOTAKA, KOBAYASHI FUMIE, YAMAGUCHI KYOJI, GOTO MASAAKI, MORINAGA TOMONORI
• Format: Patent
• Language: English
• Published: 22.01.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a new protein and a method for producing the same. SOLUTION: The new protein binds specifically to the osteoclastogenesis inhibitory factor (OCIF) and has high affinity thereto. The new protein can be obtained by preparing a cell membrane fraction from osteoblast-like cell membranes cultured in the presence of a bone absorption-accelerating factor or stroma cells derived from bone marrow, solubilizing a membrane protein by a surfactant and purifying by using an OCIF-immobilized affinity column. By using the protein, screening of physiologically active substances can be performed or an antibody against this protein can be obtained. COPYRIGHT: (C)2009,JPO&INPIT