SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREFOR

• Main Authors: OKAMURA HIROSHI, ICHIHARA TERUHISA, OBA MITSUYOSHI, NIKA MICHIFUMI, YAMANO HIROBUMI, KAMEI SHUICHI
• Format: Patent
• Language: English
• Published: 04.12.2008
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a solid support suitable to duplicating DNA by a nucleic-acid amplification reaction (polymerase chain reaction), high in the amount of nucleic-acid molecules that are immobilized, and high in bond strength of nucleic-acid molecules. SOLUTION: A solid support includes: a substrate, an electrostatic layer provided on the substrate so as to electrostatically attract nucleic acid molecules; and functional groups capable of covalently binding to nucleic acid molecules. The substrate has a surface provided with at least one surface treatment layer selected from the group consisting of diamond, soft diamond, carbonaceous matter, and carbide, with the electrostatic layer formed thereon. COPYRIGHT: (C)2009,JPO&INPIT