AUTOMATIC CELL-CULTURING APPARATUS

• Main Author: TSUMURA HISAFUMI
• Format: Patent
• Language: English
• Published: 09.10.2008
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide an automatic cell-culturing apparatus constructing a system not necessitating a CPC (Cell Processing Center) corresponding to GMC (Good Manufacturing Practice), automating cell seeding, medium exchange, quality control, etc., based on a rotating cultivation technology using a RWV (rotating wall vessel). SOLUTION: The automatic cell-culturing apparatus includes a rotational culturing device a cylindrical cultivating container 2 having a horizontal rotating shaft 14, an inlet 11 for supplying cell sap, a supply port 12 and outlet 13 for exchanging culture solution in a closed housing, in which the supply port and the outlet are provided as a pair on the outer circumferential cylindrical-face of the cultivating container at positions 180° apart from each other to the center of the rotation, and a syringe shifting means inserting/withdrawing a supply syringe 4 containing new culture solution to the supply port in airtight state and inserting/withdrawing an empty discharging syringe 5 on the outlet in airtight state, in a state setting the supply port and the outlet on a vertical line and locating, the supply port on the upper side, and piston driving means pushing in the piston of the supplying syringe and pulling out the piston of the discharging syringe are provided. COPYRIGHT: (C)2009,JPO&INPIT