CHIMERIC PROTEIN OF TISSUE FACTOR ACTIVITY INHIBITOR TFPI AND TFPI-2 AND MUTEIN

• Main Authors: INNIS MICHAEL A, CREASEY ALBA A
• Format: Patent
• Language: English
• Published: 29.05.2008
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for producing, in high yield, a protein molecule having anticoagulating activity and other activity equivalent to at least human tissue factor activity inhibitor (TFPI) when being not enhanced and decreasing immunogenicity of protein when administered to mammal and having little glycosylation moiety. SOLUTION: The present invention provides a method for producing a chimeric protein containing Kunitz-type domain 1 of TFPI-2 and Kunitz-type domain 2 of TFPI. The method comprises (a) a step for providing a transformed host cell containing a polynucleotide sequence encoding the chimeric protein and further containing an expression control sequence which is compatible with expression of the polynucleotide sequence encoding the chimeric protein and (b) a step for incubating the transformed host cell under conditions expressing the polynucleotide sequence encoding the chimeric protein. COPYRIGHT: (C)2008,JPO&INPIT