DETECTION OF DNA SEQUENCE VARIATION

• Main Authors: CASE-GREEN STEPHEN C, SOUTHERN EDWIN MELLOR, PRITCHARD CLARE E
• Format: Patent
• Language: English
• Published: 29.11.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a common method applicable to all variants generally used as a DNA marker for genetic analysis. SOLUTION: The analysis method comprises preparing a polynucleotide target involving nucleotide of variable base and one oligonucleotide probe fixed on a support which probe is complementary to the target and terminates at a position before its 5' terminal by one base; and comprises (a) incubating the target with the probe to form a duplex chain, (b) incubating the duplex chain with a labelled oligonucleotide complementary to the target including predicted variant of the target under a ligation condition, and (c) monitoring the addition of oligonucleotide to probe by ligation of the step (b) as one exhibiting point mutation at the specific position in the target. COPYRIGHT: (C)2008,JPO&INPIT