GENOME-WIDE DNA MARKER OF YEAST SACCHAROMYCES CEREVISIAE

PROBLEM TO BE SOLVED: To provide a genome-wide DNA marker by specifying microsatellite polymorphism existing in a genomic DNA of yeast Saccharomyces cerevisiae, and to provide a PCR primer set for detecting the DNA marker. SOLUTION: This genome-wide DNA marker is recognizable based on a difference i...

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Bibliographic Details
Main Authors SHIMOII HITOSHI, NAMISE MASAHIRO, HATA YOJI
Format Patent
LanguageEnglish
Published 05.04.2007
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a genome-wide DNA marker by specifying microsatellite polymorphism existing in a genomic DNA of yeast Saccharomyces cerevisiae, and to provide a PCR primer set for detecting the DNA marker. SOLUTION: This genome-wide DNA marker is recognizable based on a difference in length of a fragment of a PCR amplification product, wherein the DNA marker is developed so that a site at which the microsatellite polymorphism having a different number of cycles exists is specified in a genome-wide manner, by conducting shotgun sequence of Sake yeast Kyokai No.7 genome and being compared with a genome sequence of laboratory yeast S288C made public on the Internet, and further a primer oligonucleotide which specifically detects a gene locus exhibiting the polymorphism is designed. COPYRIGHT: (C)2007,JPO&INPIT
Bibliography:Application Number: JP20050272751