MOLECULAR MOTOR SYSTEM FOR LOADING AND UNLOADING MOLECULAR LOAD

• Main Authors: TAIRA OSAMU, ODAKA MASATO, MORI EICHIYU
• Format: Patent
• Language: English
• Published: 12.10.2006
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain a molecular motor system that immobilizes a target molecular load through hybridization to a microtube in excellent selectivity, moves the target molecular load even after loading and unloads a molecular charge since the DNA of a linker part in the loaded molecular load is cleft by a restriction enzyme. SOLUTION: The molecular motor system for loading and unloading a molecular load comprises a linker DNA conjugate microtube in which a single-stranded linker DNA is bonded to a microtube, a substrate coated with kinesin and a buffer solution, reacts a sample DNA containing a target DNA with a linker DNA conjugate microtube, immobilizes a target molecular load having a modified DNA sequence complementary to the linker DNA on a microtube to a DNA conjugate microtube by hybridization, selectively moves the objective target molecular load by moving the substrate coated with kinesin and unloads the molecular load by cleaving the linker DNA by a restriction enzyme. COPYRIGHT: (C)2007,JPO&INPIT