METHOD FOR JUDGING KIND OF HOP USING MICROSATELLITE DNA

• Main Authors: KANAI DAISUKE, KIRITA MASANOBU, SAKAMOTO KANTA
• Format: Patent
• Language: English
• Published: 09.02.2006
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a rapid/ready method for identifying main kinds recently circulated in large amounts in a hop market by carrying out a PCR of a genome DNA extracted from the hop using a primer to amplify a microsatellite region by which the kind of the hop can be identified. SOLUTION: The method for judging the kind of the hop comprises carrying out the polymerase chain reaction (PCR) of the DNA of the hop which is a specimen by using the kind-identifying primer designed so that the microsatellite DNA containing polymorphism on base sequences among kinds may be amplified to amplify the microsatellite DNA, and analyzing the amplified DNA fragments. COPYRIGHT: (C)2006,JPO&NCIPI