METHOD FOR DETECTING DNA AND/OR RNA, AND KIT FOR DETECTING MEDIATOR OLIGONUCLEOTIDE AND DNA AND/OR RNA

• Main Authors: HIRAYAMA KOICHI, OKAMURA HIROSHI, ICHIHARA TERUHISA, NIKA MICHIFUMI, YAMANO HIROBUMI, KAMEI SHUICHI
• Format: Patent
• Language: English
• Published: 26.01.2006
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for detecting DNA and/or RNA solving the problem that, in a hybridization having been conventionally used for detecting a target DNA, one of double-stranded PCR products competitively hinders the other's hybridization, therefore resulting in poor efficiency and leaving detection sensitivity low. SOLUTION: The method for detecting DNA and/or RNA in a specimen comprises contacting a mediator oligonucleotide with a to-be-detected oligonucleotide, wherein the mediator oligonucleotide is such that a 1st oligonucleotide portion specifically bindable to the to-be-detected oligonucleotide immobilized on a substrate and a 2nd oligonucleotide portion specifically bindable to the DNA and/or RNA in the specimen are joined to each other. COPYRIGHT: (C)2006,JPO&NCIPI