METHOD FOR DETECTING DNA AND/OR RNA, AND KIT FOR DETECTING MEDIATOR OLIGONUCLEOTIDE AND DNA AND/OR RNA
PROBLEM TO BE SOLVED: To provide a method for detecting DNA and/or RNA solving the problem that, in a hybridization having been conventionally used for detecting a target DNA, one of double-stranded PCR products competitively hinders the other's hybridization, therefore resulting in poor effici...
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Main Authors | , , , , , |
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Format | Patent |
Language | English |
Published |
26.01.2006
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Subjects | |
Online Access | Get full text |
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Summary: | PROBLEM TO BE SOLVED: To provide a method for detecting DNA and/or RNA solving the problem that, in a hybridization having been conventionally used for detecting a target DNA, one of double-stranded PCR products competitively hinders the other's hybridization, therefore resulting in poor efficiency and leaving detection sensitivity low. SOLUTION: The method for detecting DNA and/or RNA in a specimen comprises contacting a mediator oligonucleotide with a to-be-detected oligonucleotide, wherein the mediator oligonucleotide is such that a 1st oligonucleotide portion specifically bindable to the to-be-detected oligonucleotide immobilized on a substrate and a 2nd oligonucleotide portion specifically bindable to the DNA and/or RNA in the specimen are joined to each other. COPYRIGHT: (C)2006,JPO&NCIPI |
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Bibliography: | Application Number: JP20040202976 |