PRODUCTION OF TISSUE FACTOR ACTIVITY INHIBITOR

• Main Authors: INNIS MICHAEL A, CREASEY ALBA A
• Format: Patent
• Language: English
• Published: 22.12.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To produce a uniform mature TFPI (tissue factor potency inhibitor) having authentic nature (right N-terminal amino acid sequence) and complete length. SOLUTION: A method for producing a VIIa/TF/Xa (VIIa factor/tissue factor/Xa) factor binding protein contains (a) a step to incubate a yeast cell transformed with a replicable cloned vehicle under a condition suitable for the production of the VIIa/TF/Xa factor binding protein, wherein the replicable cloned vehicle contains the 1st nucleotide sequence encoding the VIIa/TF/Xa factor binding protein and the VIIa/TF/Xa factor binding protein is retained in the yeast cell, (b) a step to prepare an insoluble fraction of the transformed yeast cell containing the VIIa/TF/Xa factor binding protein and (c) a step to separate the VIIa/TF/Xa factor binding protein from the insoluble fraction. COPYRIGHT: (C)2006,JPO&NCIPI