PROTEIN DETECTION METHOD

PROBLEM TO BE SOLVED: To provide a protein detection method which uses a nonradioactive label, less likely to be interfered than biotin/(strepto) avidin mutual interaction, and is capable of achieving detection sensitivity which is higher than that of a detection system which uses DIG. SOLUTION: The...

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Bibliographic Details
Main Author SUDO YUKIO
Format Patent
LanguageEnglish
Published 30.06.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a protein detection method which uses a nonradioactive label, less likely to be interfered than biotin/(strepto) avidin mutual interaction, and is capable of achieving detection sensitivity which is higher than that of a detection system which uses DIG. SOLUTION: The target substance detection method includes both a process (A) for bringing a target substance into contact and bonding reaction, with a protein labeled with a compound, having a six-membered ring expressed in Formula (1) and a process (B) for detecting the protein labeled with the compound, having the six-membered ring bonded with the target substance, through the use of an antibody to the compound, having the six-membered ring. In the Formula (1), R1and R2may be the same or different from each other, each independently indicate a hydrogen atom or an alkyl group of carbon numbers 1-6, and a dotted line indicates an atomic group required for forming the six-membered ring. COPYRIGHT: (C)2005,JPO&NCIPI
Bibliography:Application Number: JP20030415269