PURIFICATION OF PLASMID DNA

PROBLEM TO BE SOLVED: To provide a purification system requiring no centrifugal separation of plasmid DNA and a method for using the same. SOLUTION: The system comprises a crude filter having about 50-200μm pore size, a prefilter having about 1-2μm pore size and a final filter having about 0.22μm po...

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Bibliographic Details
Main Author ANTONIOU CHRIS
Format Patent
LanguageEnglish
Published 16.06.2005
Edition7
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a purification system requiring no centrifugal separation of plasmid DNA and a method for using the same. SOLUTION: The system comprises a crude filter having about 50-200μm pore size, a prefilter having about 1-2μm pore size and a final filter having about 0.22μm pore size. The prefilter and the final filter are preferably combined into one filter house to reduce a system holdup volume and enhance plasmid DNA recovery. By the method, the system is operated not only continuously but also batchwisely. COPYRIGHT: (C)2005,JPO&NCIPI
Bibliography:Application Number: JP20040179365