ANISOTROPIC BIOTINYLATED LATEX MICROPARTICLE BEARING ATP-PRODUCTIVE ENZYME

• Main Authors: NAKAMURA KAZUHIKO, ODAKA MASATO, TOMOHIRO TAKENORI, MORI EICHIYU
• Format: Patent
• Language: English
• Published: 10.03.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain such anisotropic biotinylated latex microparticles that one side of a microparticle bindable to a molecular motor and capable of producing ATP needs to have a binding site for the molecular motor while the other side needs to have a binding site for an ATP-productive enzyme and these two binding sites are reactive independently of each other, and to obtain another kind of an anisotropic biotinylated latex microparticles where the epoxy groups of the above anisotropic biotinylated latex microparticles are immobilized with the enzyme. SOLUTION: The anisotropic biotinylated latex microparticles are obtained by the following process: Using anisotropic latex microparticles wherein epoxy rings and hydroxy groups are borne on the different domains of the microparticles, the hydroxy group side is reacted with an activated ester-type biotin derivative to biotinylate only one side of the microparticles. The other kind of anisotropic biotinylated latex microparticles are obtained by immobilizing the enzyme on the epoxy groups of the above anisotropic biotinylated latex microparticles. COPYRIGHT: (C)2005,JPO&NCIPI