METHOD FOR EVALUATING UPTAKE REGULATING ACTION OF CELL

PROBLEM TO BE SOLVED: To provide a method for assaying 2-deoxyglucose-6-phosphate having excellent assaying properties by which practice can be carried out in a short time and the amount of uptake of glucoses into a cell can accurately be evaluated without requiring a special facility for using a ra...

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Bibliographic Details
Main Authors MUROYAMA KOTARO, MUROZAKI SHINJI, HIROSE YOSHITAKA, YAMAMOTO YOSHIHIRO, SATO TAKUYA, YAMAMOTO KENRO
Format Patent
LanguageEnglish
Published 27.01.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a method for assaying 2-deoxyglucose-6-phosphate having excellent assaying properties by which practice can be carried out in a short time and the amount of uptake of glucoses into a cell can accurately be evaluated without requiring a special facility for using a radioisotope and without a risk of exposure to radioactivity. SOLUTION: This method for assaying the 2-deoxyglucose-6-phosphate is characterized as follows. The 2-deoxyglucose-6-phosphate in the cell is reacted with a reaction liquid containing (a) a glucose-6-phosphate dehydrogenase, (b) oxidized form nicotinamide adenine dinucleotide or oxidized form nicotinamide adenine dinucleotide phosphate, (c) a diaphorase and (d) resazurin to measure the fluorescence intensity of the produced resorufin. COPYRIGHT: (C)2005,JPO&NCIPI
Bibliography:Application Number: JP20030187614