NEW GENE INVOLVED IN FERRICHRYSIN SYNTHESIS PATHWAY AND METHOD FOR CREATING FERRICHRYSIN NON-PRODUCING KOJI MOLD USING THE SAME

PROBLEM TO BE SOLVED: To provide a koji mold with the amount of secreted and produced ferrichrysin reduced even to at least ≤1/10 times based on that of a koji mold Aspergillus oryzae RIB40 by utilizing an ornithine-N5-oxygenase gene which is a new gene involved in a ferrichrysin synthesis pathway d...

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Bibliographic Details
Main Authors AKITA OSAMU, WATANABE HISANORI, SATO TOSHIJI, EI HITOSHI, YAMADA OSAMU, AKAO TAKESHI
Format Patent
LanguageEnglish
Published 02.04.2004
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
OTHER ALCOHOLIC BEVERAGES
PREPARATION THEREOF
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a koji mold with the amount of secreted and produced ferrichrysin reduced even to at least ≤1/10 times based on that of a koji mold Aspergillus oryzae RIB40 by utilizing an ornithine-N5-oxygenase gene which is a new gene involved in a ferrichrysin synthesis pathway derived from the koji mold Aspergillus oryzae and to provide a food and liquors with a reduced content of the ferrichrysin produced by utilizing the koji mold and further reduced discoloration derived from the ferrichrysin. SOLUTION: A gene encoding the ornithine-N5-oxygenase is cloned from the koji mold to determine the base sequence thereof. The koji mold is transformed with a DNA containing a partial fragment of the gene to select a strain in which the gene disruption is caused by homologous recombination. Thereby, the koji mold with a remarkably reduced amount of the produced ferrichrysin is created. COPYRIGHT: (C)2004,JPO
Bibliography:Application Number: JP20020259915