METHOD FOR ISOLATING GPCR GENE AND NEW GPCR GENE

• Main Authors: TAKAO KYOICHI, SUGA HINAKO, TAKAO TETSUYA
• Format: Patent
• Language: English
• Published: 19.02.2004
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for highly efficiently and simply isolating a G-protein coupled receptor gene, and to provide a new G-protein coupled receptor gene. SOLUTION: This method for isolating the G-protein coupled receptor gene is characterized by comprising the following steps: (a) a step for designing a primer on the basis of the sequence information of a known G-protein coupled receptor gene or its peripheral region or on the basis of the sequence information of an estimated G-protein coupled receptor gene, (b) a step for performing an amplification reaction using the above-described designed primer and using as a template a cDNA library originated from a tissue except a tissue expressing the G-protein coupled receptor, and (c) a step for confirming whether the cloned gene has or does not have a function as the G-protein coupled receptor. COPYRIGHT: (C)2004,JPO