METHOD AND DEVICE FOR PROVIDING MEASUREMENT OF QUANTITY OF ENZYME WITH HIGH SENSITIVENESS AND WIDE MEASURING RANGE

PROBLEM TO BE SOLVED: To provide a method and a device capable of resolving a problem of an over range without physically using, for example, two detectors, and capable of detecting an enzyme even when a substrate to be converted into a detectable substance by the enzyme becomes short due to an extr...

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Bibliographic Details
Main Authors SHIMADA HIDEMI, KAI TETSUYA
Format Patent
LanguageEnglish
Published 25.09.2003
Edition7
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Summary:PROBLEM TO BE SOLVED: To provide a method and a device capable of resolving a problem of an over range without physically using, for example, two detectors, and capable of detecting an enzyme even when a substrate to be converted into a detectable substance by the enzyme becomes short due to an extremely high quantity of the enzyme to be determined. SOLUTION: In the method and device for determining the quantity of the enzyme, a reaction liquid is produced by adding the enzyme to a solution of the substrate converted into an optically measurable substance by enzyme activity of the enzyme, an inhibitor suppressing the enzyme activity of the enzyme is added to the reaction liquid, and one or more optical measurement is carried out in a state suppressing the enzyme activity of the enzyme. COPYRIGHT: (C)2003,JPO
Bibliography:Application Number: JP20020068640