CULTIVATION OF PROTOZOA

PROBLEM TO BE SOLVED: To cultivate protozoa useful for a water treatment process, research and development of medicaments and the like, and to obtain biologically homogeneous, high purity protozoa by infecting animal culture cells with cyst or oocyst of protozoa and by propagating the protozoa in th...

Full description

Saved in:
Bibliographic Details
Main Authors KATO TOSHIRO, MIKI OSAMU
Format Patent
LanguageEnglish
Published 09.01.2001
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:PROBLEM TO BE SOLVED: To cultivate protozoa useful for a water treatment process, research and development of medicaments and the like, and to obtain biologically homogeneous, high purity protozoa by infecting animal culture cells with cyst or oocyst of protozoa and by propagating the protozoa in the culture cells. SOLUTION: Protozoa are cultivated by infecting animal culture cells such as a mouse-derived BALB/3T33 cell line with cyst or oocyst of protozoa such as Cryptosporidium and by propagating the protozoa in the culture cells. In this method, the cyst or oocyst of protozoa is extracted and recovered from the culture supernatant of the cultured product by one method selected from the group consisting of (flotation) centrifugation, density gradient centrifugation, membrane separation, filtration, gel filtration and affinity chromatography or a combinational method consisting of at least two selected from the above group.
Bibliography:Application Number: JP19990175855