SCREENING OF TCP-1-CONTAINING CHAPERONIN EXPRESSION CONTROL SUBSTANCE

• Main Authors: KUBOTA HIROSHI, YURA TAKASHI, YANAGI HIDEKI
• Format: Patent
• Language: English
• Published: 28.11.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

PROBLEM TO BE SOLVED: To provide a method for screening the subject substance such as a medicine for treating a cancer by examining the expression of a reporter gene by a cell strain to which a specific vector containing TCP-1-containing chaperonin gene is transferred in the presence of a compound to be examined. SOLUTION: A vector in which a reporter gene such as luciferase gene is connected to the downstream of a promoter of TCR-1-containing chaperonin (CCT) gene such as a gene of CCTα subunit is transferred to a cell strain such as HeLa cell, to transform the cell strain. The expression of a reporter gene product is examined by using the transformed cell strain in the presence of a compound to be examined to screen an expression control substance of TCP-1- containing chaperonin (CCT). An expression inhibitor of CCT selectable by the screening is useful as a medicinal composition for treating a cancer.