TRANSCRIPTION CONTROLLING FACTOR PROTEIN

• Main Authors: KISHIMOTO TOSHIHIKO, NIWA SHINICHIRO, TAMURA TAKAAKI, MAKINO YASUTAKA
• Format: Patent
• Language: English
• Published: 02.08.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain the subject new protein having a TATA bonding function and a transcription activating function, being whose gene used as a diagnosis probe or a primer, etc., comprising a transcription controlling factor protein having a specific amino acid sequence. SOLUTION: This new transcription controlling factor protein is an amino acid sequence expressed by the formula and has a TATA bonding function and a transcription activating function, then its gene is useful for a diagnosis of a genetic disease such as a diagnosis probe for one kind of genetic disease caused from an abnormal transcription of a gene or as a primer of PCR. The transcription controlling factor protein is obtained by detecting a base sequence encoding a TATA bonding protein from a data base by using a human lever cDNA library and a human retinal cDNA library as templates, performing PCR by using the resultant designed and synthesized primer and integrating the resultant gene into a vector, then transducing into a host cell to express the gene.