MEASUREMENT OF CRYPTOSPORIDIUM PARVUM GENE BY REAL TIME DETECTION PCR METHOD AND PRIMER AND PROBE USED THEREFOR

• Main Authors: OIKAWA EISAKU, KIMURA KENJI, TAKANO YUKIE
• Format: Patent
• Language: English
• Published: 13.06.2000
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

PROBLEM TO BE SOLVED: To obtain a new forward-side primer which consists of an oligonucleotide having a specific base sequence and a specific number of bases, and is useful for the simple and sensitive determination of the gene derived from Crystosporidium parvum by the real time detection PCR method. SOLUTION: A primer, which is an oligonucleotide having a base sequence consisting of serial 15-42 bases among the base sequence shown by the formula (T may be U), is a new forward-side primer used for the determination of the gene derived from Crystospordium parvum by the real time detection PCR method, and is useful for the simple and sensitive determination of C. parvum causing human serious watery stool, gastrospasm, abdominal pain, and so on by infecting human, is obtained by designing the sequence from the base sequence of 18S rRNA gene derived from C. parvum followed by chemical synthesizing the sequence.