AQUEOUS SOLUTION AND METHOD FOR PROTEIN EXTRACTION, AND METHOD FOR ANALYZING ALLERGEN

PROBLEM TO BE SOLVED: To provide an aqueous solution and a method for protein extraction, which are capable of extracting protein even from heated food without being denatured, and to provide an analyzing method for allergen. SOLUTION: An aqueous solution for extraction contains an alkyl sulfate, ur...

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Bibliographic Details
Main Authors TSUBAKI KAZUFUMI, IKEZAWA YOSHIRO, MOGI KAZUYUKI, SUGIYAMA HIROSHI, SHIMADA SADASUKE
Format Patent
LanguageEnglish
Published 03.03.2000
Edition7
Subjects
FOODS OR FOODSTUFFS
FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J
HUMAN NECESSITIES
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
SEPARATION
TESTING
THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
TRANSPORTING
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Summary:PROBLEM TO BE SOLVED: To provide an aqueous solution and a method for protein extraction, which are capable of extracting protein even from heated food without being denatured, and to provide an analyzing method for allergen. SOLUTION: An aqueous solution for extraction contains an alkyl sulfate, urea, and dithiothreitol or 2-mercaptoethanol in the molar ratios 3.1-52:2000 or more:1.1 or more (in the case of dithiothreitol) or 9-400 (in the case of 2- mercaptoethanol). The extraction is performed in an aqueous extraction system containing 0.09-1.5 wt. % of the alkyl sulfate, 2 M or more of urea, and 1.1 mM or more of dithiothreitol or 9-400 mM of 2-mercaptoethanol. Allergen analysis is carried out for immunologically measuring the allergenition of protein in the obtained extract.
Bibliography:Application Number: JP19980232537