METODO PER LA QUANTIFICAZIONE DELLO STATO DI ATTIVAZIONE DEI RECETTORI ACCOPPIATI A PROTEINE G IN UN CAMPIONE BIOLOGICO UMANO

• Main Authors: LICCARDO, Federica, LUINI, Alberto, LO MONTE, Matteo, MARZULLO, Vincenzo Manuel
• Format: Patent
• Language: Italian
• Published: 27.11.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A method for measurement of an activation state of G-protein-coupled receptors comprises: supplying a human biological sample; preparing a fluorescent donor probe comprising an anti-GPCR Fab fragment or antibody and a fluorophore group linked via amide bond to an amino group of the N-terminal amino acid of the light chain and/or of the N-terminal amino acid of the heavy chain of the Fab fragment or antibody; preparing a fluorescent acceptor probe comprising an anti-G-protein and/or anti-arrestin Fab fragment or antibody and a fluorophore group linked via amide bond to an amino group of the N-terminal amino acid of the light chain and/or of the N-terminal amino acid of the heavy chain of the Fab fragment or antibody; setting the human biological sample in contact with the fluorescent acceptor probe and with the fluorescent donor probe; sensing the activation state of the GCPR receptors by means of fluorescence microscopy techniques.