VETTORE AD ESPRESSIONE E SECREZIONE IN LIEVITI,UTILE PER LA PREPARAZIONE DI PROTEINE ETEROLOGHE

• Main Authors: BENSI GIULIANO, PALLA EMANUELA, GALEOTTI CESIRA, RAUGEI GIOVANNI, MELLI MARIA LUISA
• Format: Patent
• Language: Italian
• Published: 16.11.1988
• Edition: 2
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Expression and secretion vector in yeasts, useful for preparing heterologous proteins, comprising a synthetic oligonucleotide, which directs the secretion of the heterologous protein, positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polyinker followed by the signals of transcription termination recognized by the RNA-polymerase of the yeasts. Hybrid plasmid obtained by cloning in one of the restriction sites of the polyinker of said vector, the DNA sequence which codes for a heterologous protein. Process for the preparation of heterologous proteins, which comprises cultivating, in a suitable culture medium, a yeast transformed with said hybrid plasmid, and recovering from the culture medium the so-obtained proteins. The so-obtained proteins comprise hormones, lymphoquins, viral antigens or immunogens, useful in the therapeutical and diagnostic field.