METODO IMMUNOMETRICO DI ANALISI COMPOSTA A REAZIONE INCROCIATA A DUE PUNTI

• Main Authors: MIRANDA QUIRINUS R, SHAH VIPIN D, KULKARNI PADMAKER V
• Format: Patent
• Language: Italian
• Published: 14.09.1988
• Edition: 4
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

In a two site cross-reaction immunoassay sandwich testing method which has a particular application in the qualitative and quantitative determination of the level of the creatine phospho- kinase MB Isoenzyme (CK-MB) in serum, there are used two different antibodies each of which is specific to a different analyte but each of which will cross-react with the analyte of interest. The first antibody which is preferably bound to a solid phase is reacted with the unknown sample. Separation of the solid and liquid portions of the first reaction mixture is accomplished and the solid portion thereof is reacted with the second antibody which is tagged. The solid and liquid portions of the second reaction mixture are separated and the solid portion is tested for the tag as an indication of the presence of said analyte. In testing for creatine phospho-kinase-MB in human serum, there are used the cross-reacting antibodies to creatine phospho-kinase-BB and to creatine phospho-kinase-MM.