ANALYTIC METHOD OF SOLID PHASE FOR DETECTING ANTIGENS

• Main Authors: RANBY,GUSTAF MATS,SE, BERGSDORF,NILS ARVID,SE
• Format: Patent
• Language: English
• Published: 30.09.1991
• Edition: 5
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A method for the quantification or qualification of antigens with an immunological solid phase method, for example of the ELISA type, during which the invention seeks to overcome the difficulties with falsely high or falsely positive measurement results. This purpose is achieved by exposing two aliquots, preferably of the same size, of the sample containing the antigen that is to be determined to two solid phases with identical anti-antigen-antibody-coated surfaces, in which one exposure (exposure 1) occurs in the presence of antibodies present in the aliquot with the same specificity as the antibodies with which the solid phases are coated, and the other exposure (exposure 2) preferably occurs in the presence of antibodies present in the aliquot with nonspecificity for the antigen in question, but otherwise essentially the same as the antibodies present in the aliquot in exposure 1, and the antigen is quantified by means of the difference between the bonded antigen in exposure 1 and the bonded antigen in exposure 2. The method is also applicable for detecting antibodies.