PROCESS FOR PRODUCING NEW DNA CLONING VECTORS AND GENE BANKS

The process entails a) removing from the cohesive 5' ends of a cloning vector, which has been isolated as linear DNA after treatment with restriction endonuclease, the phosphate groups with phosphatase, adding DNA fragments which contain the gene to be cloned, have been generated by treatment w...

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Main Authors OTT,ISTVAN,HU, VINCZE,EVA,HU, KLUPP,TIBOR,HU, MOLNAR,ISTVAN,HU, KISS,GYOERGY B.,HU, MORAVCSIK,IMRE,HU, AMBRUS,GABOR,HU, KISS,PETER,HU, SZELECZKY,ZOLTAN,HU
Format Patent
LanguageEnglish
Published 28.03.1989
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The process entails a) removing from the cohesive 5' ends of a cloning vector, which has been isolated as linear DNA after treatment with restriction endonuclease, the phosphate groups with phosphatase, adding DNA fragments which contain the gene to be cloned, have been generated by treatment with restriction endonuclease and have phosphate groups on their ends to the fragments which are incapable of joining together, and ligating the mixture with ligase, or b) cutting a recombinant cloning vector of the phage, cosmid, phasmid or transphasmid type, containing cohesive sequences, with terminase, and carrying out in vitro assembly (packaging) of the monomer DNA obtained by one of the above alternatives, and then replicating the phages.
Bibliography:Application Number: HU19870004245