METHOD TO DETERMINE ZYGOSITY OF THE FAD3 GENE IN CANOLA

The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other rel...

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Bibliographic Details
Main Authors EHLERT, Zoe, GUPTA, Manju, UBAYASENA, Lasantha, CHANNABASAVARADHYA, Chandra Shekara
Format Patent
LanguageEnglish
Hungarian
Published 28.08.2018
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
DERIVATIVES THEREOF
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
SUGARS
TRAPPING
VINEGAR
WINE
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Summary:The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.
Bibliography:Application Number: HUE12841612