PROCESS AND KIT FOR GENOTYPING CATTLE

• Main Authors: NOVOKMET, MISLAV, LAUC, GORDAN, MARJANOVI , DAMIR
• Format: Patent
• Language: Croatian; English
• Published: 10.04.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Predmetni izum odnosi se na novi multipleksni sustav za genotipizaciju goveda (Bos taurus) na osnovi 12 STR (engl. short tandem repeat) lokusa: TGLA227, BM2113, TGLA53, ETH10, SPS115, ETH3, ETH185, HAUT27, CSSM66, CSRM60, HEL9, BM1818. Određena je integrirana alelna ljestvica koja prikazuje sve alelne varijante koje se mogu pojaviti na navedenim STR lokusima (bp) ispitivane jedinke goveda:TGLA227 (79, 81, 83, 87, 90, 92, 94, 98, 100, 104);BM2113 (121, 123, 128, 131, 133, 135);TGLA53 (154, 156, 158, 160, 162, 164, 166, 168, 172, 174, 182);ETH10 (207, 211, 213, 215, 217, 219, 221, 223);SPS115 (246, 250, 252, 254, 256, 258);ETH3 (118, 120, 122, 124, 126, 128, 130, 132);ETH185 (216, 222, 223, 230, 232, 234, 236, 240);HAUT27 (134, 137, 139, 141, 145, 147, 152, 154);CSSM66 (177, 179, 181, 183, 185, 187, 191);CSRM60 (90, 93, 95, 97, 99);HEL9 (152,5; 155,5; 159,5; 161,5; 163,5; 165,5; 169,5);BM1818 (121, 123, 128, 131, 133, 135).Izum se odnosi i na set reagensa za provedbu postupka genotipizacije, te na upotrebu istog za određivanje identiteta goveda s područja zemalja bivše Jugoslavije.